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Improving Separation During Electrophoresis
12 Nov 2009: This application note discusses SeparateIT gel as a novel gel matrix for DNA electrophoresis. SeparateIT gels selectively retard the migration of large molecules, so that DNA bands remain sharp but are more spread out relative to each other. Due to this increased spacing, the resolving power of SeparateIT gels is higher compared to other gels, including polyacrylamide gels.
AppliChem GmbH
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Recent articles: |
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Performance Characterization of the FluorChem® Xplor in Imaging 2D Protein Electrophoresis Dyes and Chemiluminescent Western Blots
05 Jun 2009: The FluorChem Xplor imaging system is designed to be a total solution for the proteomics laboratory, offering rapid, high-resolution, and quantitative imaging of 1D and 2D gels, as well as chemiluminescent Western blots.
Cell Biosciences
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Removal of urea from protein samples using the NuSep MF10
19 Dec 2008: The introduction of the MF10 offers the researcher a versatile and powerful system for protein fractionation, sample preparation for 2-D PAGE, Mass Spec as well as a growing number of applications that include rapid desalting of samples, removal of charged and uncharged species from Urea or detergents and the control of denaturing conditions for proteomic studies. This application note demonstrate the removal of urea from protein samples using the MF10.
NuSep
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Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader
27 Jun 2008: The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein. As fluorescent methods are more sensitive for quantitating proteins than absorbance methods, this application note investigates a method for using Molecular Probes® NanoOrange® assay on the BMG LABTECH FLUOstar OPTIMA microplate reader.
BMG LABTECH
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ScreenTape® enables fast Quality Control of miRNA
 18 Mar 2008: The ScreenTape® System, developed by Lab901, employs a unique microscale polymer tape that contains all the reagents required for DNA electrophoresis. It brings major advantages of convenience, ease of use and safety to molecular biologists as absolutely no handling of gels and buffers is required. This application note demonstrates the benefits of using ScreenTape for qPCR QC.
Anachem Ltd (Bioscience)
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Exceptional, High Resolution Ethidium Bromide Gel Imaging
 14 Feb 2008: Ultraviolet (UV) transillumination is a ubiquitous tool in Life Science research. This application note demonstrates the use of UVP's FirstLight® Uniform UV Illuminator, GelDoc-It™ Imaging System and VisionWorks®LS Analysis Software in the quantitative analysis of DNA.
UVP, LLC.
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Chemiluminescent Western Blot Detection using the FluorChem® HD2 Imaging System
09 Nov 2007: This application note demonstrates that the detection of chemiluminescent Western blots using the FluorChem® HD2 imaging system produces high quality images and quantitative results required by the most demanding Western blot experiments.
Cell Biosciences
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G:BOX Chemi for rapid multiplex analysis - A New Method of Imaging Different Proteins using only one Blot
13 Aug 2007: Multiplex analysis of proteins can be used to determine regulation of specific proteins, to diagnose disease pre-disposition or to accurately quantify protein amounts. This application note demonstrates how the G:BOX Chemi provides a simple, yet accurate method of producing a multiplex image with up to three differently labelled proteins on the same blot.
Syngene
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Statistical Significance of Proteome Analysis
20 Dec 2006: This study compares the data sets of four large proteome studies of different biological samples with regard to the influence of the major steps – sample generation, sample preparation, protein quantification, study design, analysis, statistics – on the coefficient of variation of the whole process.
Protagen AG
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Evaluation of Different Approaches for the Chromatographic Purification of Monoclonal Antibodies
18 Dec 2006: Purification of monoclonal antibodies is one of the major applications of chromatography in the life sciences. The methods detailed in this study include: the tandem use of DEAE Affi-Gel® blue mixed mode anion exchange/dye ligand followed by Bio-Scale CHT-I ceramic hydroxyapatite chromatography column, cation exchange chromatography using a Bio-Scale S strong cation exchange column and anion exchange chromatography using a Bio-Scale DEAE weak anion exchange column.
Bio-Rad Life Sciences
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Syngene’s 2D Image Analysis Systems Being Used in Critical Research To Analyse the Efficacy of Novel Treatments for Brain Tumours 
